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64113079.pdf296.3 kB
title過重力環境がウニ単離小割球培養細胞やウニの骨片形成に与える影響
Other TitleThe effect of hypergravity on the spicule formation in the culture of sea urchin micromeres and embryos
Author(jpn)今井, 真理子; 黒谷, 明美; 江口, 星雄; 山口, 守; 清本, 正人
Author(eng)Imai, Mariko; Izumi-Kurotani, Akemi; Eguchi, Hoshio; Yamaguchi, Mamoru; Kiyomoto, Masato
Author Affiliation(jpn)お茶の水大学湾岸生物教育研究センター館山臨海実験所; 宇宙航空研究開発機構宇宙科学研究本部; 東京大学原子力研究総合センター; お茶の水大学湾岸生物教育研究センター館山臨海実験所; お茶の水大学湾岸生物教育研究センター館山臨海実験所
Author Affiliation(eng)Tateyama Marine Laboratory, Marine and Coastal Research Center, Ochanomizu University; Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA) (ISAS); Research Center for Nuclear Science and Technology, The University of Tokyo; Tateyama Marine Laboratory, Marine and Coastal Research Center, Ochanomizu University; Tateyama Marine Laboratory, Marine and Coastal Research Center, Ochanomizu University
Issue Date2006-03
PublisherInstitute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
宇宙航空研究開発機構宇宙科学研究本部
Publication titleSpace Utilization Research: Proceedings of Space Utilization Symposium
宇宙利用シンポジウム
Volume22
Publication date2006-03
Languagejpn
eng
AbstractSea urchin and other echinoderm animals have calcitic endoskeleton. In sea urchin embryo, skeletogenesis starts at late gastrula stage and then the spicles grow up to larval skeletons. This skeletogenic cells are called primary mesenchymen cells and are derived from micromeres at 16-cell stage. Spicule formation by the cultured micromere of sea urchin was used as a model for the morphogenesis in biomineralization to be adapted as an experimental system in space. We have detected an effect for spicule formation by cultured micromere in the different gravity environment. In this study we examined the effect of hypergravity in the culture medium with different serum concentration. Though the culture medium containing 4 % horse serum is suitable for the spicule formation, it was difficalt to detect significant difference in hypergravity conditon. In the low concentration of serum the number of spicule decreased and it recovered significantly in hypergravity. It was observed that extra spicules were formed later under hypergravity condition. Next, Ca(2+) transport was examined. The inhibitor of Ca(2+) - channel (diltiazem) was added to the micromere culture. The number of spicules was decreased in the culture with deltiazem. But the decrease of spicule number was recovered under hypergravity condition. These results show the possibility that the Ca(2+) uptake is one of a candidate process that is affected by hypergravity condition.
DescriptionMeeting Information: The Twenty-second Space Utilization Symposium (January 17-19, 2006: Science Council of Japan, Roppongi, Tokyo, Japan)
Meeting sponsors: The Science Council of Japan, The Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (ISAS)(JAXA)
会議情報: 第22回宇宙利用シンポジウム(2006年1月17日-19日, 日本学術会議6階会議室 六本木、東京)
共催: 日本学術会議
Keywordsskeletogenic cell, sea urchin, micromere, centrifuge, calcium, diltiazem
Document TypeConference Paper
JAXA Categoryシンポジウム・研究会
NCIDAN10324210
SHI-NOAA0064113079
URIhttps://repository.exst.jaxa.jp/dspace/handle/a-is/18299


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