JAXA Repository / AIREX 未来へ続く、宙(そら)への英知

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titleウニ胚の単離小割球培養細胞による骨片形成に対する重力環境の影響
Other TitleThe effect of gravity on the spicule formation in the culture of sea urchin micromeres
Author(jpn)今井 真理子; 清本 正人; 黒谷 明美; 江口 星雄
Author(eng)Imai, Mariko; Kiyomoto, Masato; IzumiKurotani, Akemi; Eguchi, Hoshio
Author Affiliation(jpn)お茶の水女子大学 湾岸生物教育研究センター 館山臨海実験所; お茶の水女子大学 湾岸生物教育研究センター 館山臨海実験所; 宇宙航空研究開発機構 宇宙科学研究本部; 東京大学 原子力研究総合センター
Author Affiliation(eng)Ochanomizu University Tateyama Marine Laboratory, Marine and Coastal Research Center; Ochanomizu University Tateyama Marine Laboratory, Marine and Coastal Research Center; Japan Aerospace Exploration Agency Institute of Space and Astronautical Science; University of Tokyo Research Center for Nuclear Science and Technology
Issue Date2005-03
PublisherInstitute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
宇宙航空研究開発機構宇宙科学研究本部
Publication title宇宙利用シンポジウム 第21回 平成16年度
Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium
Start page198
End page200
Publication date2005-03
Languagejpn
AbstractSpicule formation by the cultured micromere of sea urchin was used as a model for the morphogenesis in biomineralization to be adapted as an experimental system in space. Several experiments in the different gravity environment have been carried out to examine a role of gravity in the cells isolated from whole embryo and spicule elongation suppressed in the experiments by centrifugation, clinostat and a series of parabolic flight has been reported. In this study the effect of serum concentration in the culture medium on the sensitivity to hypergravity was examined. Though the culture medium containing 4 percent horse serum is suitable for the spicule formation in the micromere culture, the effect of hypergravity has been shown with various statistical significances. Lower concentration of serum was not enough for full spicule formation by the cultured micromere and the quantity of formed spicule was reduced. But the inhibitory effect of hypergravity was shown more clearly. In this new condition, it seems possible to detect the effect of smaller hypergravity. One of main contents in spicule, calcium, is incorporated from the medium in micromere culture. Higher concentration of calcium recovered spicule formation from the inhibitory effect by the hypergravity. These results show the competitive relation between hypergravity and high calcium concentration.
Keywordssea urchin; micromere; centrifuge; calcium; spicule formation; cultured cell; high gravity environment; serum; spicule quantity; horse serum; ウニ; 小割球; 遠心機; カルシウム; 骨片形成; 培養細胞; 高重力環境; 血清; 骨片量; 馬血清
Document TypeConference Paper
JAXA Categoryシンポジウム・研究会
SHI-NOAA0048095044
URIhttps://repository.exst.jaxa.jp/dspace/handle/a-is/37259


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