JAXA Repository / AIREX 未来へ続く、宙(そら)への英知

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Other TitleProteome analysis of Arabidopsis seedlings grown in differential gravity conditions
Author(jpn)鎌田 源司; 高沖 宗夫; 東谷 篤志; 石岡 憲昭
Author(eng)Kamada, Motoshi; Takaoki, Muneo; Higashitani, Atsushi; Ishioka, Noriaki
Author Affiliation(jpn)宇宙航空研究開発機構 宇宙科学研究本部; 宇宙航空研究開発機構 宇宙環境利用センター; 東北大学 大学院生命科学研究科; 宇宙航空研究開発機構 宇宙科学研究本部
Author Affiliation(eng)Japan Aerospace Exploration Agency Institute of Space and Astronautical Science; Japan Aerospace Exploration Agency Space Environment Utilization Center; Tohoku University Graduate School of Life Sciences; Japan Aerospace Exploration Agency Institute of Space and Astronautical Science
Issue Date2005-03
PublisherInstitute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
Publication title宇宙利用シンポジウム 第21回 平成16年度
Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium
Start page305
End page308
Publication date2005-03
AbstractGravity exerts a profound influence on plant growth. Proteomics may be the most promising technique to identify the proteins that are induced, repressed, or post-transcriptionally modified during gravity response in plants. In this research, the proteins controlled by gravity condition were identified. First, total protein extracted from Arabidopsis seedlings grown in 1 G-stationary and clino-rotated condition for seven days was used. Then, gravity-regulated proteins expression was analyzed by two-dimensional gel electrophoresis (2-DE). Several proteins which appeared different expressions between stationary and clino-rotated conditions were identified by Matrix Assisted Laser Desorption Ionization - Time Of Flight Mass Spectrometry (MALDI-TOFMS) analysis and Peptide Mass Fingerprinting (PMF).
Keywordsarabidopsis; gravitational effect; mass spectroscopy; proteome; two dimensional electrophoresis; gene expression; mechanoreceptor; peptide mass fingerprinting; rubisco related protein; gravitational response; clinostat; シロイヌナズナ; 重力効果; 質量分析; プロテオーム; 2次元電気泳動; 遺伝子発現; 機械受容器; ペプチド質量フィンガープリント; ルビスコ関連タンパク質; 重力応答; クリノスタット
Document TypeConference Paper
JAXA Categoryシンポジウム・研究会

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