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titleキュウリのオーキシン排出キャリア遺伝子群cDNAの単離と発現解析
Other TitleIsolation of cucumber auxin efflux carrier cDNAs and expression of corresponding mRNA in cucumber seedlings
Author(jpn)藤井 伸治; 堀田 拓哉; 鎌田 源司; 宮沢 豊; 高橋 秀幸; Kim, Dai-Hee; Kim, Kyung-Min
Author(eng)Fujii, Nobuharu; Hotta, Takuya; Kamada, Motoshi; Miyazawa, Yutaka; Takahashi, Hideyuki; Kim, Dai-Hee; Kim, Kyung-Min
Author Affiliation(jpn)東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; Kyungpook National University Inst. of Agricultural Science and Tech.; Kyungpook National University Institute of Genetic Engineering
Author Affiliation(eng)Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Kyungpook National University Institute of Agricultural Science and Technology; Kyungpook National University Institute of Genetic Engineering
Issue Date2005-03
PublisherInstitute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
宇宙航空研究開発機構宇宙科学研究本部
Publication title宇宙利用シンポジウム 第21回 平成16年度
Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium
Start page294
End page297
Publication date2005-03
Languagejpn
AbstractWhen cucumber (Cucumis sativus L.) seeds germinate in a horizontal position, a protuberance called a peg forms on the lower side of the transition zone. It has been shown that the endogenous levels of IAA that induces peg formation are significantly lower in the upper side of the transition zone than that in the lower side. To understand the mechanism of decrease of auxin in the upper side of the transition zone in response to gravity, cucumber cDNAs similar to PIN auxin efflux carrier gene were isolated and tissue specificity of their mRNA accumulation was investigated. The results indicate that mRNAs of CsPIN1, CsPIN4 and CsPIN6 accumulate in the transition zone of 24-h-old cucumber seedlings that initiate peg formation. CsPIN1 mRNA abundantly accumulates in xylem and endodermis. CsPIN4 mRNA abundantly accumulates in xylem. CsPIN6 mRNA abundantly accumulates in phloem and pith, and also accumulates in endodermis and cortex. Further study of these CsPINs will reveal the auxin transport system regulating peg formation of cucumber.
Keywordsauxin; cucumber; peg; gene expression; molecular phylogentic tree; ribonucleic acid; gravitational effect; hybridization; amino acid; base sequence; オーキシン; キュウリ; ペグ; 遺伝子発現; 分子系統樹; リボ核酸; 重力効果; ハイブリダイゼーション; アミノ酸; 塩基配列
Document TypeConference Paper
JAXA Categoryシンポジウム・研究会
SHI-NOAA0048095068
URIhttps://repository.exst.jaxa.jp/dspace/handle/a-is/51045


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