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title酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析
Other TitleProteomics study on the variation of protein expressions and phosphorylations due to oxidative stress
Author(jpn)三浦 ゆり; 加納 まゆみ; 戸田 年総; 鈴木 捷三
Author(eng)Miura, Yuri; Kano, Mayumi; Toda, Toshifusa; Suzuki, Shozo
Author Affiliation(jpn)東京都老人総合研究所; 東京都老人総合研究所; 東京都老人総合研究所; 東京都老人総合研究所
Author Affiliation(eng)Tokyo Metropolitan Institute of Gerontology; Tokyo Metropolitan Institute of Gerontology; Tokyo Metropolitan Institute of Gerontology; Tokyo Metropolitan Institute of Gerontology
Issue Date2005-03
PublisherInstitute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
宇宙航空研究開発機構宇宙科学研究本部
Publication title宇宙利用シンポジウム 第21回 平成16年度
Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium
Start page205
End page208
Publication date2005-03
Languagejpn
AbstractThe protein profiles of variously aged rat astrocytes were investigated, which responded to oxidative stress. After hydroperoxide (H2O2)-exposure of cells at 100 micro mols for 30 min, the relative intensity of 10 protein spots changed on 2-D gels compared with control gels after silver staining. MALDI-TOF/MS (Matrix-Assisted Laser Desorption/Ionization - Time Of Flight/Mass Spectrometry) analysis after in-gel digestion revealed that six of these spots corresponded to three kinds of proteins, each of which was composed of a protein and its modified form with a different isoelectric point. These three proteins were identified as Peroxiredoxins (Prxs) 2 and 3, and calpactin 1 light chain (p11). H2O2-exposure increased the intensity of the spot with lower isoelectric point and simultaneously decreased that of the spot with higher isoelectric point for both Prxs 2 and 3. In addition, the expression of annexin 7, S-Adenosyl-L-Homocysteine Hydrolase (SAHH), Elongation Factor 2 (EF-2) fragment, and Adenosine Deaminase (ADA) were increased by H2O2-exposure in glial cells from variously aged rats. Phosphorylated proteins were also analyzed using the Pro-Q Diamond staining, and it was found that Hsp (Heat shock protein) 60 and alpha-tubulin were phosphorylated by H2O2-exposure, and that the phosphorylation of alpha-tubulin was enhanced with aging.
Keywordsoxidative stress; hydroperoxide; proteomics; phosphorylation; proteome; electrophoresis; peroxiredoxin; astrocyte; stress; rat; 酸化ストレス; ヒドロペルオキシド; プロテオミクス; リン酸化; プロテオーム; 電気泳動; ペルオキシレドキシン; 星状細胞; ストレス; ラット
Document TypeConference Paper
JAXA Categoryシンポジウム・研究会
SHI-NOAA0048095046
URIhttps://repository.exst.jaxa.jp/dspace/handle/a-is/52421


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