WEKO3
アイテム
{"_buckets": {"deposit": "ed2a6ac0-ac5e-4d72-87d2-7dca323d428a"}, "_deposit": {"created_by": 1, "id": "13775", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "13775"}, "status": "published"}, "_oai": {"id": "oai:jaxa.repo.nii.ac.jp:00013775", "sets": ["1646", "1891"]}, "author_link": ["126449", "126448", "126453", "126454", "126450", "126451", "126452", "126455"], "item_5_alternative_title_2": {"attribute_name": "その他のタイトル(英)", "attribute_value_mlt": [{"subitem_alternative_title": "Proteomics study on the variation of protein expressions and phosphorylations due to oxidative stress"}]}, "item_5_biblio_info_10": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "208", "bibliographicPageStart": "205", "bibliographic_titles": [{"bibliographic_title": "宇宙利用シンポジウム 第21回 平成16年度"}, {"bibliographic_title": "Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium", "bibliographic_titleLang": "en"}]}]}, "item_5_description_17": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "The protein profiles of variously aged rat astrocytes were investigated, which responded to oxidative stress. After hydroperoxide (H2O2)-exposure of cells at 100 micro mols for 30 min, the relative intensity of 10 protein spots changed on 2-D gels compared with control gels after silver staining. MALDI-TOF/MS (Matrix-Assisted Laser Desorption/Ionization - Time Of Flight/Mass Spectrometry) analysis after in-gel digestion revealed that six of these spots corresponded to three kinds of proteins, each of which was composed of a protein and its modified form with a different isoelectric point. These three proteins were identified as Peroxiredoxins (Prxs) 2 and 3, and calpactin 1 light chain (p11). H2O2-exposure increased the intensity of the spot with lower isoelectric point and simultaneously decreased that of the spot with higher isoelectric point for both Prxs 2 and 3. In addition, the expression of annexin 7, S-Adenosyl-L-Homocysteine Hydrolase (SAHH), Elongation Factor 2 (EF-2) fragment, and Adenosine Deaminase (ADA) were increased by H2O2-exposure in glial cells from variously aged rats. Phosphorylated proteins were also analyzed using the Pro-Q Diamond staining, and it was found that Hsp (Heat shock protein) 60 and alpha-tubulin were phosphorylated by H2O2-exposure, and that the phosphorylation of alpha-tubulin was enhanced with aging.", "subitem_description_type": "Other"}]}, "item_5_description_32": {"attribute_name": "資料番号", "attribute_value_mlt": [{"subitem_description": "資料番号: AA0048095046", "subitem_description_type": "Other"}]}, "item_5_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "宇宙航空研究開発機構宇宙科学研究本部"}]}, "item_5_publisher_9": {"attribute_name": "出版者(英)", "attribute_value_mlt": [{"subitem_publisher": "Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)"}]}, "item_5_text_20": {"attribute_name": "その他キーワード", "attribute_value_mlt": [{"subitem_text_value": "ライフサイエンス(全般)"}, {"subitem_text_value": "航空宇宙医学"}]}, "item_5_text_35": {"attribute_name": "JAXAカテゴリ", "attribute_value_mlt": [{"subitem_text_value": "JAXAカテゴリ: シンポジウム・研究会"}]}, "item_5_text_36": {"attribute_name": "JAXAカテゴリ2", "attribute_value_mlt": [{"subitem_text_value": "JAXAカテゴリ2: IS"}]}, "item_5_text_42": {"attribute_name": "シンポジウム区分", "attribute_value_mlt": [{"subitem_text_value": "シンポジウム区分: 008"}]}, "item_5_text_43": {"attribute_name": "DSpaceコレクション番号", "attribute_value_mlt": [{"subitem_text_value": "DSpaceコレクション番号: 7"}]}, "item_5_text_6": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "東京都老人総合研究所"}, {"subitem_text_value": "東京都老人総合研究所"}, {"subitem_text_value": "東京都老人総合研究所"}, {"subitem_text_value": "東京都老人総合研究所"}]}, "item_5_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Tokyo Metropolitan Institute of Gerontology"}, {"subitem_text_language": "en", "subitem_text_value": "Tokyo Metropolitan Institute of Gerontology"}, {"subitem_text_language": "en", "subitem_text_value": "Tokyo Metropolitan Institute of Gerontology"}, {"subitem_text_language": "en", "subitem_text_value": "Tokyo Metropolitan Institute of Gerontology"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "三浦, ゆり"}], "nameIdentifiers": [{"nameIdentifier": "126448", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "加納, まゆみ"}], "nameIdentifiers": [{"nameIdentifier": "126449", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "戸田, 年総"}], "nameIdentifiers": [{"nameIdentifier": "126450", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鈴木, 捷三"}], "nameIdentifiers": [{"nameIdentifier": "126451", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miura, Yuri", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "126452", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kano, Mayumi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "126453", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Toda, Toshifusa", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "126454", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Shozo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "126455", "nameIdentifierScheme": "WEKO"}]}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "酸化ストレス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ヒドロペルオキシド", "subitem_subject_scheme": "Other"}, {"subitem_subject": "プロテオミクス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "リン酸化", "subitem_subject_scheme": "Other"}, {"subitem_subject": "プロテオーム", "subitem_subject_scheme": "Other"}, {"subitem_subject": "電気泳動", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ペルオキシレドキシン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "星状細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ストレス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ラット", "subitem_subject_scheme": "Other"}, {"subitem_subject": "oxidative stress", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hydroperoxide", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proteomics", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phosphorylation", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proteome", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "electrophoresis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "peroxiredoxin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "astrocyte", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "stress", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rat", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference paper", "resourceuri": "http://purl.org/coar/resource_type/c_5794"}]}, "item_title": "酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析"}]}, "item_type_id": "5", "owner": "1", "path": ["1646", "1891"], "permalink_uri": "https://jaxa.repo.nii.ac.jp/records/13775", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-03-26"}, "publish_date": "2015-03-26", "publish_status": "0", "recid": "13775", "relation": {}, "relation_version_is_last": true, "title": ["酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析"], "weko_shared_id": -1}
酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析
https://jaxa.repo.nii.ac.jp/records/13775
https://jaxa.repo.nii.ac.jp/records/137756470e182-e54d-46fc-980f-98b7ad3ad6d8
Item type | 会議発表論文 / Conference Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-03-26 | |||||
タイトル | ||||||
タイトル | 酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 酸化ストレス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ヒドロペルオキシド | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | プロテオミクス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | リン酸化 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | プロテオーム | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 電気泳動 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ペルオキシレドキシン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 星状細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ストレス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ラット | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | oxidative stress | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | hydroperoxide | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | proteomics | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphorylation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | proteome | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | electrophoresis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | peroxiredoxin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | astrocyte | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | stress | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | rat | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
資源タイプ | conference paper | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
その他のタイトル(英) | ||||||
その他のタイトル | Proteomics study on the variation of protein expressions and phosphorylations due to oxidative stress | |||||
著者 |
三浦, ゆり
× 三浦, ゆり× 加納, まゆみ× 戸田, 年総× 鈴木, 捷三× Miura, Yuri× Kano, Mayumi× Toda, Toshifusa× Suzuki, Shozo |
|||||
著者所属 | ||||||
東京都老人総合研究所 | ||||||
著者所属 | ||||||
東京都老人総合研究所 | ||||||
著者所属 | ||||||
東京都老人総合研究所 | ||||||
著者所属 | ||||||
東京都老人総合研究所 | ||||||
著者所属(英) | ||||||
en | ||||||
Tokyo Metropolitan Institute of Gerontology | ||||||
著者所属(英) | ||||||
en | ||||||
Tokyo Metropolitan Institute of Gerontology | ||||||
著者所属(英) | ||||||
en | ||||||
Tokyo Metropolitan Institute of Gerontology | ||||||
著者所属(英) | ||||||
en | ||||||
Tokyo Metropolitan Institute of Gerontology | ||||||
出版者 | ||||||
出版者 | 宇宙航空研究開発機構宇宙科学研究本部 | |||||
出版者(英) | ||||||
出版者 | Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS) | |||||
書誌情報 |
宇宙利用シンポジウム 第21回 平成16年度 en : Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium p. 205-208, 発行日 2005-03 |
|||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The protein profiles of variously aged rat astrocytes were investigated, which responded to oxidative stress. After hydroperoxide (H2O2)-exposure of cells at 100 micro mols for 30 min, the relative intensity of 10 protein spots changed on 2-D gels compared with control gels after silver staining. MALDI-TOF/MS (Matrix-Assisted Laser Desorption/Ionization - Time Of Flight/Mass Spectrometry) analysis after in-gel digestion revealed that six of these spots corresponded to three kinds of proteins, each of which was composed of a protein and its modified form with a different isoelectric point. These three proteins were identified as Peroxiredoxins (Prxs) 2 and 3, and calpactin 1 light chain (p11). H2O2-exposure increased the intensity of the spot with lower isoelectric point and simultaneously decreased that of the spot with higher isoelectric point for both Prxs 2 and 3. In addition, the expression of annexin 7, S-Adenosyl-L-Homocysteine Hydrolase (SAHH), Elongation Factor 2 (EF-2) fragment, and Adenosine Deaminase (ADA) were increased by H2O2-exposure in glial cells from variously aged rats. Phosphorylated proteins were also analyzed using the Pro-Q Diamond staining, and it was found that Hsp (Heat shock protein) 60 and alpha-tubulin were phosphorylated by H2O2-exposure, and that the phosphorylation of alpha-tubulin was enhanced with aging. | |||||
資料番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 資料番号: AA0048095046 |