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title血管内皮細胞における重力ベクトル変化に対するRho活性化因子の発現抑制とアミノ酸配列
Other TitleGene expression of Rho guanine nucleotide exchange factor in bovine brain microvascular endothelial cell under vector altered gravity and its amino acid sequence
Author(jpn)東端 晃; 佐藤今溝 真理; 関 真也; 石岡 憲昭
Author(eng)Higashibata, Akira; SatoImamizo, Mari; Seki, Masaya; Ishioka, Noriaki
Author Affiliation(jpn)宇宙航空研究開発機構 宇宙科学研究本部; エイ・イー・エス 宇宙開発部; エイ・イー・エス 宇宙開発部; 宇宙航空研究開発機構 宇宙科学研究本部
Author Affiliation(eng)Japan Aerospace Exploration Agency Institute of Space and Astronautical Science; Advanced Engineering Services Co. Ltd. Space Development Division; Advanced Engineering Services Co. Ltd. Space Development Division; Japan Aerospace Exploration Agency Institute of Space and Astronautical Science
Issue Date2005-03
PublisherInstitute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
宇宙航空研究開発機構宇宙科学研究本部
Publication title宇宙利用シンポジウム 第21回 平成16年度
Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium
Start page209
End page210
Publication date2005-03
Languagejpn
AbstractIt has been reported that actin stress fiber and microtubule were disorganized under microgravity. But the mechanism of these disorganizations is unclear. In the last symposium, it was reported that the effect of gene expression in bovine endothelial cells was detected by differential display method. Some fragments that showed different expression level were picked up and were determined the primary sequence of these. From the results of database search, one of the fragments was identified as Leukemia-Associated Rho-GEF (LARG). From the open reading frame research, LARG of bovine endothelial cells was contained the specific domains of Dbl family. The homology each domain between bovine LARG and human LARG showed high as follows: 98.8 percent in PDZ domain, 95.7 percent in LH domain, 98.6 percent in DH domain and 99.2 percent in PH domain. In this study, the fragment that showed the different expression under clinorotation as LARG was identified. It was concluded that gravity vector changing affects the signaling pathway of Rho activation.
Keywordsclinostat; gene; gene expression; deoxyribonucleic acid; clinorotation; gravitational effect; endothelial cell; vector altered gravity; differential display; open reading frame; クリノスタット; 遺伝子; 遺伝子発現; デオキシリボ核酸; 傾斜回転; 重力効果; 内皮細胞; ベクトル変化重力; ディファレンシャルディスプレイ; オープンリーディングフレーム
Document TypeConference Paper
JAXA Categoryシンポジウム・研究会
SHI-NOAA0048095047
URIhttps://repository.exst.jaxa.jp/dspace/handle/a-is/57051


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