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title重力屈性欠損アサガオ(シダレアサガオ)の変異原因遺伝子の同定とその回旋転頭運動における役割
Other TitleThe mutated gene responsible for agravitropic growth of the weeping morning glory, Shidare-asagao
Author(jpn)北澤 大典; 畑田 泰子; 藤井 伸治; 宮沢 豊; 菅 洋; 高橋 秀幸; 鎌田 源司; 星野 敦; 飯田 滋; 深城 英弘
Author(eng)Kitazawa, Daisuke; Hatakeda, Yasuko; Fujii, Nobuharu; Miyazawa, Yutaka; Suge, Hiroshi; Takahashi, Hideyuki; Kamada, Motoshi; Hoshino, Atsushi; Iida, Shigeru; Fukaki, Hidehiro
Author Affiliation(jpn)東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 東北大学 大学院生命科学研究科; 宇宙航空研究開発機構; 基礎生物学研究所; 基礎生物学研究所; 奈良先端科学技術大学院大学 バイオサイエンス研究科
Author Affiliation(eng)Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Tohoku University Graduate School of Life Sciences; Japan Aerospace Exploration Agency; National Institute for Basic Biology; National Institute for Basic Biology; Nara Institute of Science and Technology Graduate School of Biological Sciences
Issue Date2005-03
PublisherInstitute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
宇宙航空研究開発機構宇宙科学研究本部
Publication title宇宙利用シンポジウム 第21回 平成16年度
Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium
Start page298
End page300
Publication date2005-03
Languagejpn
AbstractAn agravitropic mutant of morning glory (Pharbitis nil.), weeping, has defect in shoot gravitropism. The previous results showed that weeping also showed defect in proper endodermis development and in shoot circumnutation, and such defect in circumnutation was also observed in similar endodermis-less Arabidopsis mutant, scarecrow (scr). In search of the mutated gene of weeping, cDNAs for SCR homolog from morning glory (PnSCR) were cloned from both wild type and weeping, respectively, and analyzed. An amino acid insertion was found in PnSCR from weeping. To investigate whether this insertion inactivates the PnSCR functions, complementation test was performed by introducing wild and weeping type PnSCR to the different Arabidopsis scr mutants respectively. As a result, endodermis-less and shoot agravitropic phenotypes were rescued by wild type introducing PnSCR, whereas introduction of weeping type PnSCR did not rescue them. Moreover, shoot circumnutation was also restored in scr mutant to which wild type PnSCR was transformed, but transformed weeping type PnSCR did not restore shoot circumnutation. These results strongly suggest that defects in gravitropism, proper endodermis development, and circumnutation observed in weeping are attributable to the loss of function of PnSCR. Possibility for usage of weeping morning glory as a new model plant in space biology is discussed.
Keywordscircumnutation; morning glory; gravitropism; gene; mutation; mutant; amino acid; electrophoresis; gravitation; cloning; 回旋転頭運動; アサガオ; 重力屈性; 遺伝子; 突然変異; 突然変異株; アミノ酸; 電気泳動; 重力; クローニング
Document TypeConference Paper
JAXA Categoryシンポジウム・研究会
SHI-NOAA0048095069
URIhttps://repository.exst.jaxa.jp/dspace/handle/a-is/57144


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