| タイトル | Induction of carcinoembryonic antigen expression in a three-dimensional culture system |
| 本文（外部サイト） | http://hdl.handle.net/2060/19960007785 |
| 著者（英） | Ford, R. D.; Nachman, A.; Jessup, J. M.; Goodwin, T. J.; Spaulding, G.; Brown, D.; Fitzgerald, W. |
| 著者所属（英） | NASA Johnson Space Center |
| 発行日 | 1994-01-01 |
| 言語 | eng |
| 内容記述 | MIP-101 is a poorly differentiated human colon carcinoma cell line established from ascites that produces minimal amounts of carcinoembryonic antigen (CEA), a 180 kDa glycoprotein tumor marker, and nonspecific cross-reacting antigen (NCA), a related protein that has 50 and 90 kDa isoforms, in vitro in monolayer culture. MIP-101 produces CEA when implanted into the peritoneum of nude mice but not when implanted into subcutaneous tissue. We tested whether MIP-101 cells may be induced to express CEA when cultured on microcarrier beads in three-dimensional cultures, either in static cultures as non-adherent aggregates or under dynamic conditions in a NASA-designed low shear stress bioreactor. MIP- 101 cells proliferated well under all three conditions and increased CEA and NCA production 3 - 4 fold when grown in three-dimensional cultures compared to MIP-101 cells growing logarithmically in monolayers. These results suggest that three-dimensional growth in vitro simulates tumor function in vivo and that three-dimensional growth by itself may enhance production of molecules that are associated with the metastatic process. |
| NASA分類 | LIFE SCIENCES (GENERAL) |
| レポートNO | 96N14951
NASA-TM-111144
NAS 1.15:111144
NIPS-95-06161 |
| 権利 | No Copyright |
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