AIREX: Full-length nanopore sequencing of circular RNA landscape in peripheral blood cells following sequential BNT162b2 mRNA vaccination

タイトル	Full-length nanopore sequencing of circular RNA landscape in peripheral blood cells following sequential BNT162b2 mRNA vaccination
DOI	https://doi.org/10.1016/j.gene.2024.148971
本文（外部サイト）	https://ir.library.osaka-u.ac.jp/repo/ouka/all/98596/Gene_933_148971.pdf
著者（英）	Liu, Yu Chen; Ishikawa, Masakazu; Sakakibara, Shuhei; Al Kadi, Mohamad; Motooka, Daisuke; Naito, Yoko; Ito, Shingo; Imamura, Yuko; Matsumoto, Hisatake; Sugihara, Fuminori; Hirata, Haruhiko; Ogura, Hiroshi; Okuzaki, Daisuke
発行日	2025-01-15
発行機関など	Elsevier B.V.
刊行物名	Gene
巻	933
開始ページ	148971
言語	eng
内容記述	Liu Y.C., Ishikawa M., Sakakibara S., et al. Full-length nanopore sequencing of circular RNA landscape in peripheral blood cells following sequential BNT162b2 mRNA vaccination. Gene 933, 148971 (2025); https://doi.org/10.1016/j.gene.2024.148971. Circular RNAs (circRNA) lack 5′ or 3′ ends; their unique covalently closed structures prevent RNA degradation by exonucleases. These characteristics provide circRNAs with high pharmaceutical stability and biostability relative to current standard-of-care linear mRNAs. CircRNA levels are reportedly associated with certain human diseases, making them novel disease biomarkers and a noncanonical class of therapeutic targets. In this study, the endogenous circRNAs underlying the response to BNT162b2 mRNA vaccination were evaluated. To this end, peripheral blood samples were subjected to full-length sequencing of circRNAs via nanopore sequencing and transcriptome sequencing. Fifteen samples, comprising pre-, first, and second vaccination cohorts, were obtained from five healthcare workers with no history of SARS-CoV-2 infection or previous vaccination. A total of 4706 circRNAs were detected; following full-length sequencing, 4217 novel circRNAs were identified as being specifically expressed during vaccination. These circRNAs were enriched in the binding motifs of stress granule assemblies and SARS-CoV-2 RNA binding proteins, namely poly(A) binding protein cytoplasmic 1 (PABPC1), pumilio RNA binding family member 1 (PUM1), and Y box binding protein 1 (YBX1). Moreover, 489 circRNAs were identified as previously reported miRNA sponges. The differentially expressed circRNAs putatively originated from plasma B cells compared to circRNAs reported in human blood single-cell RNA sequencing datasets. The pre- and post-vaccination differences observed in the circRNA expression landscape in response to the SARS-CoV-2 BNT162b2 mRNA vaccine.
キーワード	Blood sample; BNT162b2; circRNA; mRNA vaccine; SARS-CoV-2
権利	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
URI	https://repository.exst.jaxa.jp/dspace/handle/a-is/1297777