| タイトル | IKKε is SUMOylated by ORF45 on Lys231 and Lys549. |
| 著者（英） | Xiaoqian Wang (460263); Zhenshan Liu (147780); Xue Xu (138262); Xin Wang (91924); Zizhen Ming (20577503); Chengrong Liu (11018778); Hang Gao (1457464); Tingting Li (10553); Qiming Liang (2746603) |
| 発行日 | 2025-01-17T18:54:01Z |
| 内容記述 | <p><i>(A</i>) KSHV ORF45 promotes IKKε SUMOylation. HEK293T cells were co-transfected with indicated plasmids and cell lysates were subjected to IP under denature condition and IB with indicated antibodies. (<i>B</i>) aa 300–500 and aa 500–716 of IKKε interact with ORF45. HEK293T cells were co-transfected with indicated plasmids and cell lysates were subjected to IP and IB with indicated antibodies. (<i>C</i>) ORF45 is required for IKKε SUMOylation during KSHV lytic replication. iSLK-BAC16 and iSLK-BAC16-ORF45<sup>stop</sup> cells were induced with Dox and NaB for lytic replication. Cell lysates were harvested at 48 h post-induction and subjected to IP under denature condition and IB with indicated antibody. (<i>D</i>) Lys231 and Lys549 are the SUMOylation sites of IKKε by ORF45. HEK293T cells were co-transfected with indicated plasmids and cell lysates were subjected to IP under denature condition and IB with indicated antibodies.</p> <p>(TIFF)</p> |
| 権利 | CC BY 4.0 |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/1307569 |
