| タイトル | Positive-Type Reversibly Photoswitching Red Fluorescent Protein for Dual-Color Superresolution Imaging with Single Light Exposure for Off-Switching |
| DOI | https://doi.org/10.1021/acsnano.4c16847 |
| 本文（外部サイト） | https://ir.library.osaka-u.ac.jp/repo/ouka/all/101031/ACSNano_19_7_7188.pdf |
| 著者（英） | Ozaki-Noma, Ryohei; Wazawa, Tetsuichi; Kakizuka, Taishi; Shidara, Hisashi; Takemoto, Kiwamu; Nagai, Takeharu |
| 発行日 | 2025-02-25 |
| 発行機関など | American Chemical Society |
| 刊行物名 | ACS Nano |
| 巻 | 19 |
| 号 | 7 |
| 開始ページ | 7188 |
| 終了ページ | 7201 |
| 言語 | eng |
| 内容記述 | Ozaki-Noma R., Wazawa T., Kakizuka T., et al. Positive-Type Reversibly Photoswitching Red Fluorescent Protein for Dual-Color Superresolution Imaging with Single Light Exposure for Off-Switching. ACS Nano 19, 7188 (2025); https://doi.org/10.1021/acsnano.4c16847.
Positive-type reversibly photoswitching fluorescent proteins (p-rsFPs) transition to a bright on-state upon light exposure for fluorescence excitation and to a dark off-state under a different wavelength. p-rsFPs are widely used in superresolution (SR) imaging techniques, offering simplified observation procedure and enhanced biocompatibility. Although some green p-rsFPs possess adequate photoproperties for SR imaging, all red p-rsFPs (p-rsRFPs) to date exhibit suboptimal properties, limiting the color palette for multiplexed SR imaging. Here, we present a p-rsRFP, rsZACRO, with 3.0-fold brighter fluorescence, 5.3-fold faster off-switching, and 1.5-fold higher on/off contrast than rsCherry, a conventional representative p-rsRFP. Using rsZACRO with superresolution polarization demodulation/on-state polarization angle narrowing (SPoD-OnSPAN), we successfully demonstrated SR imaging in the red spectrum and dual-color SR imaging with a single light for off-switching, visualizing vimentin intermediate filaments and actin filaments at higher spatial resolution than the diffraction limit of light in a living mammalian cell. |
| キーワード | chromophore phenolate; fluorescence; multiple equilibria; nanoscopy; positive-type reversibly photoswitching fluorescent protein; red fluorescent protein; superresolution imaging |
| 権利 | This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/1322001 |
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