| タイトル | Development of guayule (Parthenium argentatum) research in cell culture |
| 本文（外部サイト） | http://hdl.handle.net/2060/19810009168 |
| 著者（英） | Ball, E. A. |
| 著者所属（英） | Jet Propulsion Lab., California Inst. of Tech.|California Univ. |
| 発行日 | 1981-01-01 |
| 言語 | eng |
| 内容記述 | Utilizing the lateral buds of known high rubber producing plants as explants in culture medium specifically designed to engender shoot development and to prevent callus formation, unlimited numbers of replicate plants can be produced. Each has the same genotype as the parent. This procedure has long been used to rid plants of virus, the latter generally does not occur in the embryonic tissues of the bud; it also, by virtue of its axenic nature, eliminates all microorganisms characteristic of the parent plant. Auxins were found essential to callus formation, but since the latter is known to bring about chromosomal aberrations, it was avoided. The cytokinin benzylaminopurine strongly stimulated shoot growth, and the number of regenerated buds on the inoculum was proportional to its concentration. These buds produced shoots several centimeters in length which were caused to root on medium containing indolebutyric acid. Transferred to the septic condition of soil, the plantlets were gradually brought into full sunlight where they showed a brief vegetative growth with production of mature leaves, and flowered. |
| NASA分類 | LIFE SCIENCES (GENERAL) |
| レポートNO | 81N17691
NASA-CR-163945
UCI-59913 |
| 権利 | No Copyright |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/170099 |
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