| タイトル | LC/MS Method for the Determination of Stable Isotope Labeled Promethazine in Human Plasma |
| 本文（外部サイト） | http://hdl.handle.net/2060/20060004092 |
| 著者（英） | Putcha, Lakshmi; Zuwei, Wang; Boyd, Jason; Berens, Kurt L. |
| 著者所属（英） | Wyle Life Sciences, Inc. |
| 発行日 | 2004-01-01 |
| 言語 | eng |
| 内容記述 | Promethazine (PMZ) is taken by astronauts orally (PO), intramuscularly (IM) or rectally (PR) for space motion sickness. LC/MS method was developed with off-line solid phase extraction to measure plasma concentrations of PMZ given as stable isotope-labeled (SIL) formulations by the three different routes of administration simultaneously. Samples (0.5ml) were loaded on to Waters Oasis HLB co-polymer cartridges and eluted with 1.0 mL methanol. HPLC separation of the eluted sample was performed using an Agilent Zorbax SB-CN column (50 x 2.1 mm) at a flow rate of 0.2 mL/min for 6 min. Acetonitrile/ ammonium acetate (30 mM) in water (3:2, v/v), pH 5.6 plus or minus 0.1, was used as the mobile phase for separation. Concentrations of PMZ, PMZ-d4 and PMZ-d7 and chlorpromazine (internal standard) were determined using a Micromass ZMD single quadrupole mass spectrometer with Electrospray Ionization (ESI). ESI mass spectra were acquired in positive ion mode with selected ion monitoring of [M+ H]dot plus. The method is rapid, reproducible and the assay specific parameters are listed in a table. A novel, sensitive and specific method for the measurement of PMZ and SIL PMZ in human plasma is reported. |
| NASA分類 | Aerospace Medicine |
| 権利 | No Copyright |
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