| タイトル | Influence of Space Flight Factors on the Genetic Properties of Streptomyces Lividans 66 (PIJ702) |
| 著者（英） | Pyle, B. H.; Martinson, V. G.; Goins, T. L.; Tairbekov, M. G.; Tabakov, V. Yu.; Voeikova, T. A. |
| 著者所属（英） | NASA Ames Research Center|GosNIIGenetika |
| 発行日 | 2006-07-01 |
| 言語 | eng |
| 内容記述 | Gram-positive Streptomyces bacteria display genetic instability in response to external factors. Strain S. lividans 66 harbors the multicopy plasmid pIJ702 with selective and differential marker genes for antibiotic thiostrepton resistance and melanin production. Culture plates of modified ISP agar medium with and without thiostrepton were flown on Foton-M2. Suboptimal flight temperatures, which were simulated for asynchronous ground controls, resulted in slow growth and failure to differentiate and sporulate. Flight samples and asynchronous controls showed a high frequency of failing to express plasmid markers compared to laboratory controls. This was associated with loss of plasmid DNA and likely resulted from suboptimal temperatures for flight cultures and controls. Neither restriction fragment length polymorphism, nor polymerase chain reaction amplification coupled with denaturing gradient gel electrophoresis, revealed differences between pIJ702 DNA from flight vs. control clones. Mutations of the plasmid marker genes resulting from specific spaceflight factors, e.g., microgravity and radiation, were not detected. |
| NASA分類 | Aerospace Medicine |
| 権利 | Copyright |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/275889 |
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