タイトル | Yeast respond to hypotonic shock with a calcium pulse |
著者(英) | Schulz, T.; Masson, P. H.; Batiza, A. F. |
発行日 | 1996-09-20 |
言語 | eng |
内容記述 | We have used the transgenic AEQUORIN calcium reporter system to monitor the cytosolic calcium ([Ca2+]cyt) response of Saccharomyces cerevisiae to hypotonic shock. Such a shock generates an almost immediate and transient rise in [Ca2+]cyt which is eliminated by gadolinium, a blocker of stretch-activated channels. In addition, this transient rise in [Ca2+]cyt is initially insensitive to 1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), an extracellular calcium chelator. However, BAPTA abruptly attenuates the maintenance of that transient rise. These data show that hypotonic shock generates a stretch-activated channel-dependent calcium pulse in yeast. They also suggest that the immediate calcium influx is primarily generated from intracellular stores, and that a sustained increase in [Ca2+]cyt depends upon extracellular calcium. |
NASA分類 | Life Sciences (General) |
権利 | Copyright |
URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/296908 |
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