| タイトル | Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties |
| 著者（英） | Orihuela, C. J.; Watson, D. A.; Janssen, R.; Robb, C. W.; Niesel, D. W. |
| 発行日 | 2000-10-01 |
| 言語 | eng |
| 内容記述 | We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro. |
| NASA分類 | Life Sciences (General) |
| 権利 | Copyright |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/298696 |
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