| タイトル | Phytochrome regulates GTP-binding protein activity in the envelope of pea nuclei |
| 著者（英） | Roux, S. J.; Memon, A. R.; Thompson, G. A. Jr; Clark, G. B. |
| 発行日 | 1993-01-01
1993 |
| 言語 | eng |
| 内容記述 | Three GTP-binding proteins with apparent molecular masses of 27, 28 and 30 kDa have been detected in isolated nuclei of etiolated pea plumules. After LDS-PAGE and transfer to nitrocellulose these proteins bind [32P]GTP in the presence of excess ATP, suggesting that they are monomeric G proteins. When nuclei are disrupted, three proteins co-purify with the nuclear envelope fraction and are highly enriched in this fraction. The level of [32P]GTP-binding for all three protein bands is significantly increased when harvested pea plumules are irradiated by red light, and this effect is reversed by far-red light. The results indicate that GTP-binding activity associated with the nuclear envelope of plant cells is photoreversibly regulated by the pigment phytochrome. |
| NASA分類 | Life Sciences (General) |
| 権利 | Copyright |
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