| タイトル | Green Fluorescent Protein as a Model for Protein Crystal Growth Studies |
| 著者（英） | Pusey, Marc; Karr, Laurel; Smith, Lori; Agena, Sabine |
| 著者所属（英） | NASA Marshall Space Flight Center |
| 発行日 | 1998-01-01
1998 |
| 言語 | eng |
| 内容記述 | Green fluorescent protein (GFP) from jellyfish Aequorea Victoria has become a popular marker for e.g. mutagenesis work. Its fluorescent property, which originates from a chromophore located in the center of the molecule, makes it widely applicable as a research too]. GFP clones have been produced with a variety of spectral properties, such as blue and yellow emitting species. The protein is a single chain of molecular weight 27 kDa and its structure has been determined at 1.9 Angstrom resolution. The combination of GFP's fluorescent property, the knowledge of its several crystallization conditions, and its increasing use in biophysical and biochemical studies, all led us to consider it as a model material for macromolecular crystal growth studies. Initial preparations of GFP were from E.coli with yields of approximately 5 mg/L of culture media. Current yields are now in the 50 - 120 mg/L range, and we hope to further increase this by expression of the GFP gene in the Pichia system. The results of these efforts and of preliminary crystal growth studies will be presented. |
| NASA分類 | Solid-State Physics |
| 権利 | No Copyright |
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