| タイトル | Inhibition of the ATPase from Halobacterium Saccharovorum by Thiol Inhibitors: Evidence for the Presence of More Than One Essential Cysteinyl Residue |
| 著者（英） | Stan-Lotter, Helga; DeVincenzi, Donald L.; Hochstein, Lawrence I.; Emrich, Errol |
| 著者所属（英） | NASA Ames Research Center |
| 発行日 | 1995-01-01 |
| 言語 | eng |
| 内容記述 | The vacuolar-like ATPase from Halobacterium saccha vorum is inhibited by N-ethylmaleimide and p-chloromercudphenylsulfonate. The failure of adenine nucleotides to protect against p-chloromercuriphenyisulfonate inhibition, of p-chloromercuriphenylsulfonate to protect against N-ethylmaleimide inhibition, and the difference in the temperature dependence of inactivation infers that the enzyme contains at least two thiols that are essential for enzyme activity. CNBr cleavage of C-14-N-ethylmaleimide labeled subunit results in two radioactive peptides that locates the N-ethylmaleimide-reactive cysteinyl residue as cysteine-262 in the H. salinarium sequence. |
| NASA分類 | Life Sciences (General) |
| 権利 | No Copyright |
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