| タイトル | R6 Hexameric Insulin Complexed with m-Cresol or Rescorcinol |
| 著者（英） | Rose, M. Franklin; Ciszak, Ewa; Smith, G. David; Magrum, Lucy A. |
| 著者所属（英） | Universities Space Research Association |
| 発行日 | 2000-01-01 |
| 言語 | eng |
| 内容記述 | The structures of three R6 human insulin hexamers have been determined. Crystals of monoclinic m-cresol/insulin, monoclinic resorcinol/insulin, and rhombohedral m-cresol/insulin crystals diffracted to 1.9, 1.9 and 1,78 Angstroms, respectively, and have been refined to residuals of 0.195, 0.179, and 0.200, respectively. In all three structures, a phenolic derivative is found to occupy the phenolic binding site where it forms hydrogen bonds to the carbonyl oxygen of A6 Cys and the nitrogen of A11 Cys. Two additional phenolic derivative binding sites were identified within or between hexamers. The structures of all three hexamers are nearly identical although a large displacement of the N-terminus of one B-chain in both monoclinic structures results from coordination to a sodium ion which is located between symmetry related hexamers. Other minor differences in structure are a consequence of differences in packing in the monoclinic cell as compared to the rhombohedral cell. Based upon the differences in conformation of the B13 Glu side chains in T6, T3R3, and R6 hexamers, the deprotonation of these side chains appears to be associated with the T (right arrow) R conformational transition. |
| NASA分類 | Life Sciences (General) |
| 権利 | No Copyright |
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