タイトル | Isoelectric focusing analysis of antibody clonotype changes occurring during immune responses using immobilized pH gradients |
著者(英) | Rodkey, L. Scott; Knisley, Keith A. |
著者所属(英) | Texas Univ. |
発行日 | 1988-01-01 |
言語 | eng |
内容記述 | Serum was collected from rabbits at 2-day intervals following a single injection with tetanus toxoid or at weekly intervals following multiple injections with Micrococcus lysodeikticus cell walls. These sera were analyzed for the presence of individual clonotypes of specific antitetanus or antimicrococcal antibodies by isoelectric focusing in immobilized pH gradients with added carrier ampholytes followed by affinity immunoblotting. The affinity immunoblots obtained clearly defined both the rapid disappearance and late appearance of distinct subsets of antibody clonotypes during the response. These data demonstrate the application of affinity immunoblotting combined with immobilized pH gradients for detecting the subtle changes in specific antibody clonotype patterns which occur during an immune response. |
NASA分類 | LIFE SCIENCES (GENERAL) |
レポートNO | 89A19846 |
権利 | Copyright |
URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/366369 |