| タイトル | Continuous flow electrophoretic separation of proteins and cells from mammalian tissues |
| 著者（英） | Barlow, Grant H.; Farrington, Mary Ann; Lanham, J. Wayne; Blaisdell, Steven J.; Feldmeier, Mary; Cleveland, Carolyn; Snyder, Robert S.; Grindeland, Richard; Hymer, W. C.; Hatfield, J. Michael |
| 著者所属（英） | NASA Marshall Space Flight Center|NASA Ames Research Center|Pennsylvania State Univ. |
| 発行日 | 1987-01-01 |
| 言語 | eng |
| 内容記述 | This paper describes an apparatus for continuous flow electrophoresis (CFE), designed to separate macromolecules and cells at conditions of microgravity. In this CFE, buffer flows upward in a 120-cm long flow chamber, which is 16-cm wide x 3.0-mm thick in the microgravity version (and 6-cm wide x 1.5-mm thick in the unit-gravity laboratory version). Ovalbumin and rat serum albumin were separated in space (flight STS-4) with the same resolution of the two proteins achieved at 25 percent total w/v concentration that was obtained in the laboratory at 0.2 percent w/v concentration. Rat anterior pituitary cells, cultured human embryonic kidney cells, and canine Langerhans cells were separated into subpopulations (flight STS-8) more effectively than in unit gravity, with comparable resolution having been achieved at 100 times the concentration possible on earth. |
| NASA分類 | INORGANIC AND PHYSICAL CHEMISTRY |
| レポートNO | 88A28698 |
| 権利 | Copyright |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/372091 |
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