| タイトル | Affinity immunoblotting - High resolution isoelectric focusing analysis of antibody clonotype distribution |
| 著者（英） | Rodkey, L. Scott; Knisley, Keith A. |
| 著者所属（英） | Texas Univ. |
| 発行日 | 1986-01-01 |
| 言語 | eng |
| 内容記述 | A sensitive and specific method is proposed for the analysis of specific antibody clonotype changes occurring during an immune response and for comparing multiple sera for antibody clonotype similarities. Polyclonal serum antibodies separated by isoelectric focusing (IEF) were analyzed by an affinity immunoblotting method using antigen-coated nitrocellulose membranes. Antibodies present on the surface of the acrylamide gels following IEF bind the antigen on the nitrocellulose when the coated nitrocellulose is laid over the gels. The technique has been used to analyze Ig clonotypes specific for five protein antigens and two carbohydrate antigens. Optimal antigen concentrations for coating the nitrocellulose membranes were found to range from 10-100 microgram/ml. |
| NASA分類 | LIFE SCIENCES (GENERAL) |
| レポートNO | 88A20279 |
| 権利 | Copyright |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/373515 |
