| タイトル | Isoelectric focusing-affinity immunoblot analysis of mouse monoclonal antibodies to the four human IgG subclasses |
| 著者（英） | Hamilton, Robert G.; Rodkey, L. Scott; Roebber, Marianne; Reimer, Charles B. |
| 著者所属（英） | Texas Univ.|Center for Disease Control |
| 発行日 | 1987-03-01 |
| 言語 | eng |
| 内容記述 | Isoelectric focusing (IEF)/affinity immunoblotting and enzyme-linked immunosorbent assay (ELISA) were used for parallel analysis of murine monoclonal antihuman IgG-subclass antisera (MoAbs). Coomassie Blue-stained protein bands in the pH region 5.5-8.0 were shown to be murine IgG by direct blotting onto nitrocellulose followed by detection with conjugated antimouse IgG. Use of IgG myeloma antigen-coated nitrocellulose in the IEF-affinity immunoblot allowed detection of the charge microheterogeneity of MoAbs. The MoAb group contained one to five major dense bands flanked by up to four minor fainter bands, all with pIs ranging from 6.1 to 7.8. Semiquantitative estimates of binding specificity in the IEF-affinity blot compared well with cross-reactivity data obtained from a quantitative ELISA. |
| NASA分類 | INORGANIC AND PHYSICAL CHEMISTRY |
| レポートNO | 88A14896 |
| 権利 | Copyright |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/374170 |
