タイトル | Electron transport in Paracoccus halodenitrificans and the role of Ubiquinone |
著者(英) | Cronin, S. E.; Hochstein, L. I. |
著者所属(英) | NASA Ames Research Center |
発行日 | 1984-01-01 |
言語 | eng |
内容記述 | The membrane-bound NADH oxidase of Paracoccus halodenitrificans was inhibited by dicoumarol, 2-n-heptyl-4-hydroxyquinoline-N-oxide (HQNO), and exposure to ultraviolet light (at 366 nm). When the membranes were extracted with n-pentane, NADH oxidase activity was lost. Partial restoration was achieved by adding the ubiquinone fraction extracted from the membranes. Succinate oxidation was not inhibited by dicoumarol or HQNO but was affected by ultraviolet irradiation or n-pentane extraction. However, the addition of the ubiquinone fraction to the n-pentane-extracted membranes did not restore enzyme activity. These observations suggested the reducing equivalents from succinate entered the respiratory chain on the oxygen side of the HQNO-sensitive site and probably did not proceed through a quinone. |
NASA分類 | LIFE SCIENCES (GENERAL) |
レポートNO | 84A46550 |
権利 | Copyright |
URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/396497 |
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