| タイトル | Homologous recombination of mouse ZAKI-4 gene to disrupt its expression |
| その他のタイトル | 遺伝子発現を破壊するためのマウスZAKI-4遺伝子の相同的組替え |
| 著者（日） | 加納 安彦; 阿部 直紀; 石田 純治; 深水 昭吉; 妹尾 久雄; 村田 善晴 |
| 著者（英） | Kano, Yasuhiko; Abe, Naoki; Ishida, Junji; Fukamizu, Akiyoshi; Seo, Hisao; Murata, Yoshiharu |
| 著者所属（日） | 名古屋大学環境医学研究所 分子・細胞適応部門 発生・遺伝分野; 名古屋大学環境医学研究所 分子・細胞適応部門 発生・遺伝分野; 筑波大学 筑波先端学際領域研究センター; 筑波大学 筑波先端学際領域研究センター; 名古屋大学環境医学研究所 分子・細胞適応部門 内分泌・代謝分野; 名古屋大学環境医学研究所 分子・細胞適応部門 発生・遺伝分野 |
| 著者所属（英） | Research Institute of Environmental Medicine, Nagoya University Dept. of Teratology and Genetics, Division of Molecular and Cellular Adaptation; Research Institute of Environmental Medicine, Nagoya University Dept. of Teratology and Genetics, Division of Molecular and Cellular Adaptation; University of Tsukuba Center for Tsukuba Advanced Research Alliance; University of Tsukuba Center for Tsukuba Advanced Research Alliance; Research Institute of Environmental Medicine, Nagoya University Dept. of Endocrinology and Metabolism, Div. of Molecular and Cellular Adaptation; Research Institute of Environmental Medicine, Nagoya University Dept. of Teratology and Genetics, Division of Molecular and Cellular Adaptation |
| 発行日 | 2002-12 |
| 刊行物名 | Environmental Medicine
Environmental Medicine |
| 巻 | 46 |
| 号 | 1/2 |
| 開始ページ | 55 |
| 終了ページ | 57 |
| 刊行年月日 | 2002-12 |
| 言語 | eng |
| 抄録 | ZAKI-4 inhibits the activity of calcineurin, a Ca(sup 2+)-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, alpha and beta are generated by an alternative splicing. In adult mice ZAKI-4 alpha mRNA was mainly expressed in brain whereas Z4KI-4 beta mRNA was ubiquitously. To elucidate the specific function of ZAKI-4 isoforms, it is planned to establish ZAKI-4 beta knock out mice by homologous recombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 beta sequence was disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwent homologous recombination at the ZAKI-4 beta locus. These clones will be used to establish the knock out mice. |
| キーワード | ZAKI-4 gene; homogeneous recombination; mouse; gene expression; calcineurin; mRNA; cDNA; embryonic stem cell; antibiotic; ZAKI-4遺伝子; 相同的組替え; マウス; 遺伝子発現; カルシノイリン; mRNA; cDNA; 胚性幹細胞; 抗生物質 |
| 資料種別 | Technical Report |
| ISSN | 0287-0517 |
| SHI-NO | AA0045052012 |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/42067 |
