| タイトル | ML-9 inhibits methacholine-induced C[lc]a(2+) mobilization in guinea pig tracheal smooth muscle |
| その他のタイトル | ML-9はモルモットの気管平滑筋におけるメタコリン誘起Ca(2+)移動性を阻害する |
| 著者（日） | 伊藤 康; 久米 裕昭; 加藤 秀樹; 本荘 晴朗; 山木 健市; 児玉 逸雄 |
| 著者（英） | Ito, Satoru; Kume, Hiroaki; Kato, Hideki; Honjo, Haruo; Yamaki, Kenichi; Kodama, Itsuo |
| 著者所属（日） | 名古屋大学 医学部 第2内科; 名古屋大学 医学部 第2内科; 名古屋大学環境医学研究所 器官系機能調節部門 液性調節分野; 名古屋大学環境医学研究所 器官系機能調節部門 液性調節分野; 名古屋大学 医学部 第2内科; 名古屋大学環境医学研究所 器官系機能調節部門 循環器分野 |
| 著者所属（英） | Nagoya University Second Department of Internal Medicine, School of Medicine; Nagoya University Second Department of Internal Medicine, School of Medicine; Research Institute of Environmental Medicine, Nagoya University Department of Humoral Regulation, Division of Regulation of Organ Function; Research Institute of Environmental Medicine, Nagoya University Department of Humoral Regulation, Division of Regulation of Organ Function; Nagoya University Second Department of Internal Medicine, School of Medicine; Research Institute of Environmental Medicine, Nagoya University Department of Circulation, Division of Regulation of Organ Function |
| 発行日 | 2001-12 |
| 刊行物名 | Environmental Medicine
Environmental Medicine |
| 巻 | 45 |
| 号 | 1 |
| 開始ページ | 45 |
| 終了ページ | 47 |
| 刊行年月日 | 2001-12 |
| 言語 | eng |
| 抄録 | The effects of ML-9, a myosin light chain kinase inhibitor, on Ca(2+) mobilization and contraction induced by Methacholine (MCh) in guinea pig tracheal smooth muscle was examined. Isometric tension and intracellular Ca(2+) concentration ((Ca(2+))(sub i)) assessed by Ca(2+) indicator fura-2 were measured simultaneously. MCh (1 micro M) induced a sustained increase in (Ca(2+))(sub i) and in tension. ML-9 (100 micro M) reduced both (Ca(2+))(sub i) and contraction by MCh. There was an apparent discrepancy in the time course of reduction of between (Ca(2+))(sub i) and tension. These results suggest that ML-9 reduced MCh-induced increases in (Ca(2+))(sub i) not by MLCK inhibition but by direct effects on Ca(2+) entry in guinea pig tracheal smooth muscle. |
| キーワード | myosin light chain kinase; ML-9; calcium ion mobilization; guinea pig; tracheal smooth muscle; methacholine; inhibitor; phosphorylation; MLCK; ミオシン軽鎖キナーゼ; ML-9; カルシウムイオン移動性; モルモット; 気管平滑筋; メタコリン; 阻害剤; りん酸化; MLCK |
| 資料種別 | Technical Report |
| ISSN | 0287-0517 |
| SHI-NO | AA0032700013 |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/49680 |
