| タイトル | Comparative Analysis of Thaumatin Crystals Grown on Earth and in Microgravity |
| 著者（英） | Giege, Richard; Ng, Joseph D.; Greenwood, Aaron; Koszelak, Stanley; Day, John; Lorber, Bernard; McPherson, Alexander |
| 著者所属（英） | Centre National de la Recherche Scientifique |
| 発行日 | 1998-08-01 |
| 言語 | eng |
| 内容記述 | The protein thaumatin was studied as a model macromolecule for crystallization in microgravity environment experiments conducted on two U.S. Space Shuttle missions (second United States Microgravity Laboratory (USML-2) and Life and Microgravity Spacelab (LMS)). In this investigation we evaluated and compared the quality of space- and Earth-grown thaumatin crystals using x-ray diffraction analysis and characterized them according to crystal size, diffraction resolution limit, and mosaicity. Two different approaches for growing thaumatin crystals in the microgravity environment, dialysis and liquid-liquid diffusion, were employed as a joint experiment by our two investigative teams. Thaumatin crystals grown under a microgravity environment were generally larger in volume with fewer total crystals. They diffracted to significantly higher resolution and with improved diffraction properties as judged by relative Wilson plots. The mosaicity for space-grown crystals was significantly less than for those grown on Earth. Increasing concentrations of protein in the crystallization chambers under microgravity lead to larger crystals. The data presented here lend further support to the idea that protein crystals of improved quality can be obtained in a microgravity environment. |
| NASA分類 | Solid-State Physics |
| 権利 | No Copyright |
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