Electrophysiological properties of cultured neonatal rat ventricular myocytes over long periods

Abstract

The electrophysiological properties of single cultivated neonatal rat ventricular myocytes were investigated over long periods. The Action Potential Duration (APD) became gradually shorter during the two weeks of primary culture, resulting in a brief plateau and delayed repolarization in 15-day cells. Compared with five-day cells, both the APD(sub 25) and APD(sub 80) had shortened by 63 percent and 44 percent respectively in the 15-day cells. These results suggest that cultured neonatal rat ventricular myocytes can undergo the cell differentiation in respects of ion currents in the culture medium.

標記特性を長期間にわたって検討した。その結果、活動電位持続期間(APD)は、1次培養の2週間の間に次第に短くなり、15日齢細胞における短いプラトーと遅発性再分極を示した。5日齢細胞との比較ではAPD25およびAPD80の双方とも、15日齢ではそれぞれ63％、44％短くなった。これらの結果、培養した新生ラット心室筋細胞が、培養培地におけるイオン電流に関して細胞分化を行い得ることが示された。