| タイトル | Analysis of interleukin-8 gene promoter function in human osteoblast-like cells: Regulation by C[lc]a(2+)-signaling and cyclosporin A |
| その他のタイトル | ヒト骨芽細胞類似体におけるインターロイキン-8遺伝子プロモータ作用の解析:Ca(2+)シグナリングとシクロスポリンAによる制御 |
| 著者（日） | 光山 浩人; 神部 福司; 村上 隆一郎; 石黒 直樹; 妹尾 久雄 |
| 著者（英） | Mitsuyama, Hirohito; Kanbe, Fukushi; Murakami, Ryuichiro; Ishiguro, Naoki; Seo, Hisao |
| 著者所属（日） | 名古屋大学環境医学研究所 分子・細胞適応部門 内分泌・代謝分野; 名古屋大学環境医学研究所 分子・細胞適応部門 内分泌・代謝分野; 名古屋大学 医学部 医学科 第2内科; 名古屋大学 医学部 医学科 整形外科学; 名古屋大学環境医学研究所 分子・細胞適応部門 内分泌・代謝分野 |
| 著者所属（英） | Research Institute of Environmental Medicine, Nagoya University Dept. of Endocrinology and Metabolism, Div. of Molecular and Cellular Adaptation; Research Institute of Environmental Medicine, Nagoya University Dept. of Endocrinology and Metabolism, Div. of Molecular and Cellular Adaptation; Nagoya University Department of Internal Medicine 2, School of Medicine, Faculty of Medicine; Nagoya University Department of Orthopedics, School of Medicine, Faculty of Medicine; Research Institute of Environmental Medicine, Nagoya University Dept. of Endocrinology and Metabolism, Div. of Molecular and Cellular Adaptation |
| 発行日 | 2002-12 |
| 刊行物名 | Environmental Medicine
Environmental Medicine |
| 巻 | 46 |
| 号 | 1/2 |
| 開始ページ | 52 |
| 終了ページ | 54 |
| 刊行年月日 | 2002-12 |
| 言語 | eng |
| 抄録 | It was previously reported that an increase in intracellular calcium by calcium ionophore (A23187) and 4beta-phorbol-12beta-myristate-13alpha-acetate (PMA) induced the expression of IL-8 mRNA in human osteoblast-like HOS-TE85 cells and this induction was markedly suppressed by cyclosporin A (CsA). In this study it was investigated whether the regulation by A23187, PMA and CsA was occurred at a transcriptional level by reporter gene assays. A promoter region spanning from -1460 to +40 of IL-8 gene was PCR-amplified and inserted upstream of a luciferase reporter gene. Various deletion mutants were also constructed. Transfection of the plasmids into HOS-TE85 cells demonstrated that the nucleotides between -133 and -60 base pairs upstream of IL-8 gene are essential and sufficient for its induction by A23187/PMA, and suppression of this induction by CsA. |
| キーワード | interleukin-8; osteoblast cell; promoter function; calcium ion signaling; cyclosporin A; gene expression; transcription; reporter gene; インターロイキン-8; 骨芽細胞; プロモータ作用; カルシウムイオン・シグナリング; シクロスポリンA; 遺伝子発現; 転写; レポーター遺伝子 |
| 資料種別 | Technical Report |
| ISSN | 0287-0517 |
| SHI-NO | AA0045052011 |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/54780 |
