| タイトル | In-Flight Microbial Monitor |
| 本文（外部サイト） | http://hdl.handle.net/2060/20150016086 |
| 著者（英） | Zeitlin, Nancy; Ruby, Anna Maria; Wheeler, Raymond M.; Mullenix, Pamela |
| 著者所属（英） | NASA Kennedy Space Center |
| 発行日 | 2015-08-15 |
| 言語 | eng |
| 内容記述 | Previous research has shown that potential human pathogens have been detected on the International Space Station (ISS). New microorganisms are introduced with every exchange of crew and cargo. Microorganisms introduced to the ISS are readily transferred between crew and subsystems (i.e., ECLSS, environmental control and life support systems). Current microbial characterization methods require a culture-based enrichment of microorganisms and at least a 48-hour incubation time. This increases the microbial load while detecting only a limited number of microorganisms. The culture-based method detects approximately 1-10% of the total organisms present and provides no identification. To identify and enumerate ISS samples requires that the microbes be returned to Earth for complete analysis. Therefore, a more expedient, low-cost, inflight method of microbial detection, identification, and enumeration is needed. The RAZOR EX, a ruggedized, commercial off the shelf, real-time PCR field instrument was tested for its ability to detect microorganisms at low concentrations within one hour. Escherichia coli, Salmonella enterica Typhimurium, and Pseudomonas aeruginosa were detected at low levels using real-time DNA amplification. Total heterotrophic counts could also be detected using a 16S gene marker that can identify up to 98% of all bacteria. To reflect viable cells found in the samples, RNA was also detectable using a modified, single-step reverse transcription reaction. |
| NASA分類 | Life Sciences (General); Man/System Technology and Life Support; Aerospace Medicine |
| レポートNO | KSC-E-DAA-TN25553 |
| 権利 | No Copyright |
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