Affinity of bio-trace elements to cell component: In vitro study using multitracer

Abstract

The affinity of bio-trace metal ions to DNA (Deoxyribonucleic Acid) from liver, lung and colon of the normal and Se-deficient rat has been studied in order to clarify the possibility of distribution of metal ions in nucleic acid and the interaction of metal ions within in vivo redox system. The procedures carried out for the examination on the affinity of bio-trace metal ions in the tissue of model rats were described in details. Fe, Zn, Co, Mn and Se out of the various elements included in the multitracer solution have been analyzed, and the relative affinity of these bio-trace elements to the DNA from liver, lung and colon of the normal and Se-deficient rats were shown in figures. It was found that the affinity of metal ions decreased with DNA modification by reactive oxygen species in Se-deficient rat's DNA, and that the affinities of the liver and lung DNA were almost the same and relatively low. Possible reasons for the above results of the examination were discussed.

核酸内の金属イオン分配の可能性およびin vivo酸化還元系内の金属イオンの相互作用を明らかにするため、正常なラットおよびSe欠乏症ラットの肝臓、肺、結腸から採取したDNA(デオキシリボ核酸)に対する生体微量金属イオンの親和力を研究した。モデルラットの生体組織内の生体微量金属イオンの親和力の試験方法を詳細に記述した。多重トレーサ溶液に含まれる各種元素の中からFe、Zn、Co、Mn、Seを分析し、正常なラットとSe欠乏症ラットの肝臓、肺、結腸のDNAに対するこれらの生体微量元素の相対親和力を図示した。金属イオンの親和力はSe欠乏症ラットのDNAでは反応性酸素種によるDNA変異に伴い低下し、肝臓および肺のDNAの親和力はほとんど同じでかつ比較的低いことが分かった。試験結果に対する可能な根拠を論じた。