In vivo behavior of various elements in hepatopathic animals. 1: Metabolic study of trace elements in acute alcoholic mice

Abstract

The trace element dysbolism was studied using a model mouse having hepatopathy caused by acute alcoholism. The ddY strain mice of seven-weeks age were used for this experiment. Three ddY mice were intraperitoneally treated with alcohol at three hours before and for each eight hours from three to 71 hours after the injection of multitracer solution. Each mouse was administered by 0.025 ml/g body weight of 25 (V/V) percent at each time. The uptake of Be, Sc, Mn, Se, Rb, Y, and Zr by the liver of the acute alcoholic hepatopathy mouse was larger than that by the normal one at 24 hours after. The uptake of Be, Cr and Y by the liver of the acute alcoholic hepatopathy mouse was about three times larger than that by the normal one. Although this tendency was observed even 72 hours after the multitracer administration, the uptake of Be and Y by the liver of the acute alcoholic hepatopathy mouse became especially about 8.5 times larger than that of the normal one. In vivo, the alcohol seemed to give some effects on three-valences elements such as Sc, Cr, Mn, Y and Zr because of its larger uptake. The bone seeking elements, Be and Y, are easy to accumulate in the liver which the hepatopathy is induced. The uptake of As by the liver of the alcoholism model mouse decreased remarkably. The hemoglobin concentration in the alcoholism model mouse was 9.23 +/- 0.23 g/dl, and that in the normal mouse was 13.21 +/- 0.56 g/dl. The uptake of As by the liver seemed to decrease, since the number of the erythrocyte which transports it to the liver decreases.

急性アルコール中毒による肝機能障害を持つモデルマウスを使って、微量元素の代謝異常を調べた。7週齢になったddY系統のマウスを実験に用いた。3匹のddYマウスにマルチトレーサ溶液の注入3時間前と3?71時間後までの8時間毎に腹膜を通してアルコール処置をした。毎回25(V/V)%のアルコールを0.025ml/(g体重)だけ与えた。24時間後のBe、Sc、Mn、Se、Rb、Y、Zrの取込量は、急性アルコール中毒マウスの肝臓の方が正常のマウスより多く、Be、Cr、Yの取込量は正常のマウスの約3倍であった。この傾向は72時間後も見られたが、Be、Yは特別に約8.5倍になった。生体内で、Sc、Cr、Mn、YおよびZnなどの3価の元素は取込量が大きいため、アルコールが影響を与えているようである。向骨性元素であるBeおよびYは障害を誘発する肝臓に蓄積しやすい。アルコール中毒マウスの肝臓ではAsの取込量は著しく減少した。ヘモグロビン濃度は正常のマウスの13.21±0.56g/dlに対してアルコール中毒マウスでは9.23±0.23g/dlである。肝臓に輸送する赤血球数が減少するため、肝臓におけるAs取込量が減少するものと思われる。