タイトル | 界面活性剤の逆ミセル中におけるタンパク質の構造: 逆ミセルに可溶化した水(water poor)の異常性 |
その他のタイトル | Protein Structures in Surfactant Reverse Micelles: Abnormality of Water Incorporated in Reverse Micelles |
著者(日) | 竹田, 邦雄; 森山, 佳子 |
著者(英) | Takeda, Kunio; Moriyama, Yoshiko |
著者所属(日) | 岡山理科大学; 岡山理科大学 |
著者所属(英) | Okayama University of Science; Okayama University of Science |
発行日 | 2015-12-28 |
発行機関など | 岡山理科大学 Okayama University of Science |
刊行物名 | 岡山理科大学紀要: 自然科学 The Bulletin of Okayama University of Science: Natural sciences |
号 | 51A |
開始ページ | 31 |
終了ページ | 40 |
刊行年月日 | 2015-12-28 |
言語 | jpn eng |
抄録 | The secondary structural change and the tertiary structural change of bovine serum albumin (BSA) in the reverse micelles of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) have been examined mainly by the measurements of circular dichroism, time-resolved fluorescence, tryptophan fluorescence, and dynamic light scattering. The diameter of the reverse micelle increases with an increase of water content (W(0)=[H2O]/[AOT]). The diameter of the reverse micelle, containing BSA, is larger than the empty reverse micelle at each W(0). Upon the incorporation of BSA into the reverse micelles, the helical structures are disrupted, the fluorescence lifetime of N-iodoacetyl-N'-(5-sulfo-l-naphthyl) ethylenediamine bound to Cys34 shortens, and the maximal wavelength of tryptophan fluorescence shifts from 340 to 330 nm. These changes substantially agree with those observed in the aqueous AOT solution, indicating that the interaction between the hydrophilic groups of AOT and the oppositely charged amino acid residues of BSA plays an important role in the reverse micelle as well as the abnormality of water. |
内容記述 | 形態: 図版あり Physical characteristics: Original contains illustrations |
キーワード | surfactant; reverse micelle; protein structure; Sodium bis(2-ethylhexyl) sulfosuccinate; Bovine serum albumin; circular dichroism; dynamic light scattering |
資料種別 | Departmental Bulletin Paper |
NASA分類 | Inorganic, Organic and Physical Chemistry |
ISSN | 0285-7685 |
NCID | AN00033244 |
SHI-NO | AA1640017005 |
URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/575297 |