JAXA Repository / AIREX 未来へ続く、宙(そら)への英知
title界面活性剤の逆ミセル中におけるタンパク質の構造: 逆ミセルに可溶化した水(water poor)の異常性
Other TitleProtein Structures in Surfactant Reverse Micelles: Abnormality of Water Incorporated in Reverse Micelles
Author(jpn)竹田, 邦雄; 森山, 佳子
Author(eng)Takeda, Kunio; Moriyama, Yoshiko
Author Affiliation(jpn)岡山理科大学; 岡山理科大学
Author Affiliation(eng)Okayama University of Science; Okayama University of Science
Issue Date2015-12-28
Publisher岡山理科大学
Okayama University of Science
Publication title岡山理科大学紀要: 自然科学
The Bulletin of Okayama University of Science: Natural sciences
Issue51A
Start page31
End page40
Publication date2015-12-28
Languagejpn
eng
AbstractThe secondary structural change and the tertiary structural change of bovine serum albumin (BSA) in the reverse micelles of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) have been examined mainly by the measurements of circular dichroism, time-resolved fluorescence, tryptophan fluorescence, and dynamic light scattering. The diameter of the reverse micelle increases with an increase of water content (W(0)=[H2O]/[AOT]). The diameter of the reverse micelle, containing BSA, is larger than the empty reverse micelle at each W(0). Upon the incorporation of BSA into the reverse micelles, the helical structures are disrupted, the fluorescence lifetime of N-iodoacetyl-N'-(5-sulfo-l-naphthyl) ethylenediamine bound to Cys34 shortens, and the maximal wavelength of tryptophan fluorescence shifts from 340 to 330 nm. These changes substantially agree with those observed in the aqueous AOT solution, indicating that the interaction between the hydrophilic groups of AOT and the oppositely charged amino acid residues of BSA plays an important role in the reverse micelle as well as the abnormality of water.
Description形態: 図版あり
Physical characteristics: Original contains illustrations
Keywordssurfactant; reverse micelle; protein structure; Sodium bis(2-ethylhexyl) sulfosuccinate; Bovine serum albumin; circular dichroism; dynamic light scattering
Document TypeDepartmental Bulletin Paper
NASA Subject CategoryInorganic, Organic and Physical Chemistry
ISSN0285-7685
NCIDAN00033244
SHI-NOAA1640017005
URIhttps://repository.exst.jaxa.jp/dspace/handle/a-is/575297


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