| タイトル | An Autonomous System for Experimental Evolution of Microbial Cultures: Test Results Using Ultraviolet-C Radiation and Escherichia Coli. |
| 本文（外部サイト） | http://hdl.handle.net/2060/20170004361 |
| 著者（英） | Gentry, Diana; Wang, Jonathan; Arismendi, Dillon; Ouandji, Cynthia; Lee, Alonzo; Blaich, Justin |
| 著者所属（英） | Universities Space Research Association |
| 発行日 | 2017-04-24 |
| 言語 | eng |
| 内容記述 | At its core, the field of microbial experimental evolution seeks to elucidate the natural laws governing the history of microbial life by understanding its underlying driving mechanisms. However, observing evolution in nature is complex, as environmental conditions are difficult to control. Laboratory-based experiments for observing population evolution provide more control, but manually culturing and studying multiple generations of microorganisms can be time consuming, labor intensive, and prone to inconsistency. We have constructed a prototype, closed system device that automates the process of directed evolution experiments in microorganisms. It is compatible with any liquid microbial culture, including polycultures and field samples, provides flow control and adjustable agitation, continuously monitors optical density (OD), and can dynamically control environmental pressures such as ultraviolet-C (UV-C) radiation and temperature. Here, the results of the prototype are compared to iterative exposure and survival assays conducted using a traditional hood, UV-C lamp, and shutter system. |
| NASA分類 | Life Sciences (General) |
| レポートNO | ARC-E-DAA-TN39321 |
| 権利 | Copyright, Distribution as joint owner in the copyright |
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