| タイトル | New Techniques for Studying Calcium Gradients |
| 著者（英） | Tsien, R. Y. |
| 著者所属（英） | California Univ. |
| 発行日 | 1985-04-01 |
| 言語 | eng |
| 内容記述 | Improved techniques for detecting and manipulating spatial gradients of cytosolic free Ca(2+) concentrations (Ca(2)) sub i and intracellular stores are discussed. Three areas of progress are: (1) development of new fluorescent indicators for Ca(2+) that are the first to be suitable for measuring Ca(2+)) sub i and its inhomogeneities in individual cells; (2) invention of photolabile chelators which shift irreversibly from high to low affinity for Ca(2+) upon illumination, permitting light-controlled jumps in (Ca(2+)) sub i; and (3) fixation methods to trap dynamic intraorganellar Ca stores in a form readily visible by electron microscopy. |
| NASA分類 | LIFE SCIENCES (GENERAL) |
| レポートNO | 85N26076 |
| 権利 | No Copyright |
| URI | https://repository.exst.jaxa.jp/dspace/handle/a-is/68899 |
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